Phage display phd thesis

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In vivo phage display screenings by intravenous injection of a random phage-displayed peptide library allow for the selection of peptides that localize to specific vascular beds. At the University of Texas MD Anderson Cancer Center, we have had the opportunity to perform phage display screenings in cancer patients in order to select for cancer specific targets directly in humans. These targets serve to define biochemical diversity of endothelial cell surfaces and can be validated and explored towards the design of vascular-targeted pharmacology. In the most recent patient screen, samples were recovered from hepatocellular carcinoma HCC as well as 26 additional tissues.
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Doctoral theses

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"Mapping the Human Vasculature by In Vivo Phage Display" by Julianna Bronk

Identification of peptides capable of targeting immature dendritic cells using phage display. The ability of dendritic cells DC to be either tolerogenic or immunogenic offers many opportunities to manipulate DC function and genetically modify DC to promote allograft acceptance. However, the greatest challenge in DC gene therapy is the difficulty of achieving successful transfection. At present, DC-targeting gene delivery strategies are hindered by the lack of DC-specific target molecules. Therefore, the essential aim of this study is to isolate and identify peptides capable of targeting immature DC iDC , in particular those that internalise well as they are likely to be more effective in gene delivery.
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Application of phage display to the study of toxin-receptor interactions

Smith, Mathew Wayne Phage display and experimental brain therapeutics. PhD Thesis, Cardiff University. Phage display, a powerful polypeptide display technology, affords the rapid identification of peptides and proteins that interact with a target of interest The aims of the project were the phage display identification of peptides that interact with a druggable target in a brain disorder glioblastoma multiforme and the identification of peptides that serve as targeting vectors for brain delivery. Validation studies were undertaken to qualify the use of a cyclic 7-mer peptide phage library against targets including streptavidin and paracetamol chosen as examples of a large complex and small simple molecule, respectively.
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